Welcome!
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| Matthew E. Brown | Liupei Huang |
The Humanized Mouse Core of the University of Wisconsin-Madison provides investigators with a variety of humanized mouse models for their individual research needs. Our goal is to readily supply animals of high quality and consistency in order to facilitate cutting-edge research of the human immune response. Under the guidance of Director, Dr. Matthew Brown, the Core provides a variety of humanized animal models and consultation services to investigators.
The Humanized Mouse Core specializes in production of the current standard model in the field — the humanized immune mouse (HIM) model — including the NeoThy and BLT models incorporating the engraftment of cryopreserved human thymus tissue and hematopoietic stem cells into immune-deficient mouse host strains. Core personnel closely follow the research literature and also experimentally validate new iterations of humanized mouse models, including validating novel animal strains and the use of pluripotent stem cell-derived tissues.
Custom orders and/or collaborations are possible to establish models tailored to investigators’ individual research needs. Please contact us (brownm@surgery.wisc.edu) for additional details.
To place an order or initiate services, an iLab account is required. First time Core users, please register for iLab with the following link: https://my.ilabsolutions.com/account/signup
| Matthew E. Brown, Ph.D. |
| Director |
| Hours | Location |
| By appointment only. |
H4/725 CSC |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Matthew E. Brown |
Core Director
|
608-263-1727
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brownm@surgery.wisc.edu
|
H4/749 CSC
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| Service List |
| ► Cells (3) | |||
| Name | Description | Price | |
|---|---|---|---|
| Cord Blood CD34+ Cells (1e6) |
MACS CD34+ enriched human hematopoietic cells, no HLA typing |
UW
$1,292.82
each
External Academic $2,262.44 each Industry $2,262.44 each |
|
| Cord Blood CD34+ Cells (1e6)--HLA typed |
MACS CD34+ enriched human hematopoietic cells, HLA typed. |
UW
$1,971.91
each
External Academic $3,450.84 each Industry $3,450.84 each |
|
| PBLs (20e6 viable) |
Peripheral blood mononuclear cells (aka peripheral blood leukocytes) |
UW
$159.62
each
External Academic $279.34 each Industry $279.34 each |
|
| ► Mice (9) | |||
| Name | Description | Price | |
| Age Matched 12w |
Age matched mice, 12w old (matched to hu mouse order, if needed). |
UW
$53.25
each
External Academic $93.19 each Industry $93.19 each |
|
| Age Matched 16w |
Age matched mice, 16w old (matched to hu mouse order, if needed) |
UW
$61.73
each
External Academic $108.02 each Industry $108.02 each |
|
| Age Matched 20w |
Age matched mice, 20w old (matched to hu mouse order, if needed) |
UW
$70.20
each
External Academic $122.85 each Industry $122.85 each |
|
| Age Matched 24w |
Age matched mice, 24w old (matched to hu mouse order, if needed) |
UW
$78.68
each
External Academic $137.68 each Industry $137.68 each |
|
| BLT |
BLT type humanized mouse, created with fetal tissues |
UW
$588.00
each
External Academic $1,028.99 each Industry $1,028.99 each |
|
| Neo Thy Alt#1 |
When mice are provided by user and housed outside Core for entire Expt |
UW
$530.33
each
|
|
| NeoThy |
NeoThy humanized mouse, created with neonatal tissue |
UW
$571.26
each
External Academic $999.71 each Industry $999.71 each |
|
| NSG-Hu |
NSG-Hu humanized mouse, created with CD34+ injection alone (no thymus fragment) |
UW
$433.53
each
External Academic $758.68 each Industry $758.68 each |
|
| PBL |
PBL type humanized mouse, created with PBL injection |
UW
$292.14
each
External Academic $511.24 each Industry $511.24 each |
|
| ► Services (4) | |||
| Name | Description | Price | |
| Blood collection |
Mouse blood collection into anticoagulent. |
UW
$35.31
/hr
External Academic $61.78 /hr Industry $61.78 /hr |
|
| Custom Services |
Please inquire, email brownm@surgery.wisc.edu. |
UW
$35.31
/hr
External Academic $61.78 /hr Industry $61.78 /hr |
|
| Injections |
I.V., I.P., I.M. injections. |
UW
$35.31
/hr
External Academic $61.78 /hr Industry $61.78 /hr |
|
| Tissue Acquisition |
Mouse tissue collection e.g., kidney, liver, etc. |
UW
$35.31
/hr
External Academic $61.78 /hr Industry $61.78 /hr |
|
| ► Testing (3) | |||
| Name | Description | Price | |
| Flow Cytometry |
Chimerism Testing--1 included with each mouse order, up to 15 mice (includes bleeds, processing, etc) |
UW
$457.44
each
External Academic $800.52 each Industry $800.52 each |
|
| Flow Cytometry (Custom Panel), up to 15 mice (includes consult re panel design) |
Chimerism Testing--bleeds, processing, flow, etc. |
UW
$779.88
each
External Academic $1,364.79 each Industry $1,364.79 each |
|
| HLA typing (includes gDNA processing) |
HLA typing of HLA-A, -B,-C, -DRB1 |
UW
$643.78
each
External Academic $1,126.62 each Industry $1,126.62 each |
|
| ► Tissue (1) | |||
| Name | Description | Price | |
| Thymus fragments (cryo-preserved) |
Neonatal human thymus fragments (20) for humanization. |
UW
$187.63
each
External Academic $328.35 each Industry $328.35 each |
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